DNA
2ABH

Part:BBa_K4138000:Design

Designed by: Carlos Classen   Group: iGEM22_Aachen   (2022-09-28)


2ABH - Phosphate Binding Protein


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 645
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

We considered codon optimization for our desired E. coli strain when back-translating the amino acid sequence.


Source

We took the amino acid sequence from the Protein Data Base and back-translated it into a nucleotide sequence [1].

References

[1] https://www.rcsb.org/structure/2ABH (Source Last accessed 29.09.2022)